Figure 3.

The SH3^AGS recognition to their targets with the high-affinity binding. (A) Sequence alignment of the SH3 domains of ASAP, GRAF and SKAP proteins showing their identical Cxx(D/E) sequence in RT-loop. The unique cystine and negatively charged residue are labeled by green circles and asterisks. (B) Sequence alignment of the PRM-containing proteins targeted by ASAP, GRAF and SKAP proteins indicating an atypical class-II PRM motif. The unique positively charged residue is highlighted in orange and labeled by orange asterisk. (C) ITC-based affinity measurements of between the SH3^AGS domains and their PRM^Px+P targets.