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HPLC analysis of phenylthiohydantoin (PTH) amino acids from Edman Degradation. (A) N-terminal sequencing of linear Z3 protein. The positions of N-terminal amino acids, assigned for cycle 1 through cycle 5, are labeled. (B) N-terminal sequencing of cyclic Z3 protein, the amino acids identified are labeled from the recovered non-cyclized sequences (SSSDV) of splicing intermediates.
