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. 2023 Jan 9;24(2):1281. doi: 10.3390/ijms24021281

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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CPDY digestion SDS-PAGE analysis. Lane 1: protein ladder (Spectra Multi Color Low Range; Thermo scientific); lane 2: undigested linear Z3; lane 3: digested linear Z3 (Protein: CPDY mass ratio = 2.5:1); lane 4: undigested cyclic Z3; lane 5: digested cyclic Z3 (Protein: CPDY mass ratio = 2.5:1); lane 6: CPDY enzyme.