Table 1.
Melting temperatures (Tm) and thermodynamic parameters of unfolding (Gibbs free energy, enthalpy, and entropy change) of rituximab and an equimolar mixture of rituximab with Z3 protein variants.
| Components | Tm1 (°C) | Tm2 (°C) | Tm3 (°C) | ΔuG0 kJ mol−1 |
ΔuS0 kJ mol−1 K−1 |
ΔuH0 kJ mol−1 |
|---|---|---|---|---|---|---|
| Rituximab | 68.5 ± 0.1 | 74.1 ± 0.0 | 81.3 ± 0.5 | 55.8 ± 0.5 | 1.3 ± 0.0 | 429.9 ± 1.6 |
| Rituximab: linear Z3 (1:1) | 73.4 ± 0.1 | 84.6 ± 0.1 | N/A | 68.7 ± 3.3 | 1.4 ± 0.1 | 481.1 ± 22.6 |
| Rituximab: cyclic Z3 (1:1) | 73.4 ± 0.2 | 82.8 ± 0.1 | N/A | 66.8 ± 1.0 | 1.4 ± 0.0 | 476.0 ± 9.0 |
The standard error was calculated from three independent measurements.