Table 2.
Main functions of the differentially expressed miRNAs in SMA with nusinersen therapy.
| miRNA | Log Fold Change | p-Value | Function | PubMed Unique Identifier |
|---|---|---|---|---|
| miR-7-5p | −3.22 | 1.47 × 10−8 | Negative regulation of sprouting angiogenesis, mRNA binding involved in posttranscriptional gene silencing |
27431648, 31501273 |
| miR-15a-5p | −3.25 | 5.22 × 10−5 | Enhances cell viability and inhibits apoptosis | 32384924 |
| miR-15b-5p | −3.63 | 0.0003 | Promotes neurogenesis and inhibits neural progenitor proliferation promotes proliferation, decreases apoptosis | 20584895 32165184 |
| miR-15b-3p | −2.67 | 4.18 × 10−9 | ||
| miR-126-5p | −2.32 | 0.0005 | Promotes angiogenesis and neurogenesis, master regulator of NMJ function, Linked to neuronal loss, neurodegeneration and apoptosis in primary cultured spinal neurones |
29773756 29773756 27748416 |
| miR-127-3p | −1.87 | 0.0001 | Activate autophagy in the cortical neurons | 33723216 |
| miR-130a-3p | −2.43 | 1.35 × 10−7 | Regulate neurotransmitter synthesis | 17855557 |
| miR-146b-5p | −1.81 | 8.84 × 10−5 | Motor neuron loss caused by astrocyte-mediated pathology through NFkB signaling | 28637335 |
| miR-183-5p | −1.76 | 1.04 × 10−5 | Protein synthesis; axonal outgrowth | 29160009, 24523674, 25055867, 26459109 |
| miR-196b-5p | −1.30 | 0.0022 | Confines the rostrocaudal axis in the neural tube | 20553899 |
| miR-324-3p | −1.20 | 9.28 × 10−5 | Promotes neuronal differentiation and neurite outgrowth | 23527072 |
| miR-338-3p | −1.52 | 5.54 × 10−8 | Acts as a negative regulator of neuronal differentiation by suppressing apoptosis-associated tyrosine kinase and cytochrome oxidase complex IV | 19020050 18684991 |
| miR-378a-3p | −1.59 | 0.0003 | Promotes differentiation and inhibits proliferation of myoblasts in skeletal muscle development | 27661135 |
| miR-424-5p | −1.88 | 8.65 × 10−6 | Suppress microglia activation, regulation of cellular activities via interacting with specific lncRNA sponges | 32065781 |
The filtering was informed by a comprehensive literature search using a constellation of single and combined key search terms in PubMed including ‘motor neuron development’, ‘neurodegeneration’, ‘spinal muscular atrophy’, ‘survival motor neuron protein’ and ‘nusinersen’. A negative log fold change indicates downregulation of miRNA in the follow-up phase of nusinersen therapy.