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. 2023 Jan 8;24(2):1227. doi: 10.3390/ijms24021227

Figure 1.

Figure 1

Effect of leukotriene receptor antagonists on IL-25, IL-33, and TSLP expression. A549 human lung epithelium cells or HBE cells in air-liquid interface cultures were incubated with solvent control (Con), montelukast, or zafirlukast (0.1–10 μM) for 3 h. The supernatant for IL-25, IL-33, and TSLP protein measurement were collected after treatment for 24 h in A549 cells or 96 h in ALI cultures. The mRNA expression of IL25 (A), IL33 (B), and TSLP (C) was increased by montelukast or zafirlukast in A549 cells (n = 5). The protein levels of IL-25, IL-33, and TSLP by montelukast or zafirlukast treatment were increased in A549 cells (D,E), (n = 4) and ALI cultures (F,G), (n = 4). The expression of mRNA and protein of IL-25, IL-33, and TSLP was detected using real-time PCR or ELISA. Means ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001 compare with solvent control (Con).