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. 2023 Jan 8;24(2):1227. doi: 10.3390/ijms24021227

Figure 4.

Figure 4

Montelukast-induced increase in IL-25, IL-33, and TSLP expression by NF-κB inhibitor. A549 cells were pretreated with BAY117085 (BAY) for 1 h and stimulated with montelukast for another 3 h. The supernatant for IL-25, IL-33, and TSLP protein measurement were collected after treatment for 24 h in A549 cells or 96 h in ALI cultures. The montelukast-induced changes in the mRNA (A–C), (n = 5) or protein (D–F), (n = 4) expression of IL-25, IL-33, and TSLP were suppressed by BAY. The time course of phospho-p65 levels (G) were determined at 0, 0.5, 1, 3, and 6 h with montelukast treatment in A549 cells (n = 3). The protein levels of IL-25, IL-33, and TSLP by montelukast treatment were decreased by BAY in ALI cultures (H), (n = 4). Montelukast-induced phospho-p65 (I) levels were increased in ALI cultures (n = 3). The mRNA and protein expression of IL-25, IL-33, and TSLP was detected using real-time PCR or ELISA. The level of the p65 protein was detected using Western blotting. means ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001 compare with solvent control (Con); # p < 0.05, ## p < 0.01, and ### p < 0.001 compare with montelukast alone (Mon).