FIG. 1.

Production and secretion of Fha44-TbpB hybrid proteins by B. pertussis. Fifty-microliter samples of unconcentrated culture supernatants from B. pertussis BPGR4 containing pNFNT (lanes a), pNFCT (lanes b), pNFT/B16B6 (lanes c), or pNFT/M982 (lanes d) were analyzed by SDS-PAGE and Coomassie blue staining (A), by immunoblotting using rat anti-Fha44 (B) or rabbit anti-TbpB (C) antibodies directed against TbpB from either strain B16B6 (lanes a to c, panel C) or strain M982 (lane d, panel C), and by hTf binding overlay assays (D). The molecular masses of the chimeras are given in kilodaltons in the left margin.