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Production and secretion of the hybrid protein Fha44-TbpB/B16B6 by B. pertussis. Twenty microliters of unconcentrated culture supernatants from B. pertussis BPSM (lanes a) or BPGR4 (lanes b) and from recombinant strains BPIC3 (lanes c) or BPIC4 (lanes d) was analyzed by immunoblotting using anti-Fha44 IgY (A) or monoclonal anti-TbpB/B16B6 (B) antibody 511F8. The numbers at left are molecular masses in kilodaltons.
