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Anti-TbpB serum IgG isotype profiles elicited after i.n. administration of B. pertussis BPIC1, BPIC3, or BPIC4. Groups of three 4-week-old female OF1 mice were infected i.n. with 5 × 106 CFU of B. pertussis BPIC1 (hatched bars), BPIC3 (black bars), or BPIC4 (white bars). Five and eight weeks later, the sera were analyzed by ELISA for the presence of specific anti-TbpB IgG, IgG1, IgG2a, and IgG2b isotypes. Results are expressed as means ± standard deviations.
