TABLE 1.
B. pertussis strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| BPSM | Smr Nalr Tohama I derivative | 31 |
| BPGR4 | BPSM derivative lacking fhaB | 27 |
| BPRA | BPSM derivative lacking ptx | 4 |
| BPIC1 | BPSM derivative producing Fha44-TbpB/M982 | This study |
| BPIC3 | BPRA derivative producing Fha44-TbpB/B16B6 | This study |
| BPIC4 | BPSM derivative expressing Fha44-TbpB/B16B6 | This study |
| Plasmids | ||
| pTG4710 | pARA13 derivative containing tbpB/B16B6 | 45 |
| pTG4714 | pARA13 derivative containing tbpB/M982 | 45 |
| pTG6705 | pTG4710 derivative with tbpB mutations (R185–200) | 45 |
| pTG6706 | pTG4710 derivative with tbpB mutations (R306–321) | 45 |
| pBG4 | pBBR122 derivative with the 5′ end of fhaB (fha44) | 39 |
| pRIT13202 | pUC8 derivative containing fhaB | 10 |
| pIC-20H | ColE1-based cloning vector | 28 |
| pUC18 | ColE1-based cloning vector | 48 |
| pNFNT | pBG4 derivative with 5′ end of tbpB/B16B6 | This study |
| pNFCT | pBG4 derivative with 3′ end of tbpB/B16B6 | This study |
| pNFT/B16B6 | pBG4 derivative with tbpB/B16B6 | This study |
| pNFT/M982 | pBG4 derivative with tbpB/M982 | This study |
| pIC5 | pIC-20H derivative with fha44-tbpB/M982 | This study |
| pIC6 | pIC5 derivative with 2.4-kb BamHI fhaB fragment | This study |
| pIC7 | pUC18 derivative with 2.4-kb BamHI fhaB fragment | This study |
| pIC8 | pIC7 derivative with fha44-tbpB/B16B6 | This study |