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. 2023 Jan 6;28(2):583. doi: 10.3390/molecules28020583

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of HLE on bone formation and oxidative stress in Aβ-injured osteoblastic MC3T3-E1 cells. (A) The bone mineralization nodule determined by Alizarin red staining; (B) intracellular ROS levels determined by flow cytometry; (C–E) relative expression of Nrf2, HO-1, NQO1, FxoO1 and SOD-2 determined by Western blotting (n = 3). (# p < 0.05, ## p < 0.01, compared with the CON group; * p < 0.05, ** p < 0.01 compared with the model group. Model group means osteoblasts only treated with Aβ).