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Dose-dependent antibody responses. CBA/N mice were immunized with the indicated amounts of pJB100 or 50 μg of pNGVL3 (control). Serum samples collected on day 28 were analyzed by ELISA to determine the antibody responses. Antibody concentrations were calculated based on a standard curve generated using an anti-PspA antiserum of known concentration. Data represent the results for four mice per group. Error bars show standard errors of the means.
