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. 2023 Jan 13;24(2):1641. doi: 10.3390/ijms24021641

Figure 6.

Figure 6

Dynamics of ALI development in mice after LPS challenge. (A) Experimental setup. Mice (n = 6) were intranasally (i.n.) challenged with LPS (10 µg per mouse) followed by collecting bronchial alveolar lavage (BAL) fluid and lung tissue at 1, 4, 8, 16, and 24 h after induction. (B) Total leukocytes counts (left panel) and the level of pro-inflammatory cytokine IL6 measured by ELISA (right panel) in the BAL fluid of LPS-challenged mice. Four BAL samples from each experimental group were analyzed. (C) The expression levels of Timp1 (left panel) and Mmp9 (right panel) in the lung tissue of LPS-challenged mice measured by RT-qPCR. Expression levels were normalized to the expression level of Tbp (for Timp1) and Hprt (for Mmp9) used as reference genes. The level in control, non-treated cells was set as 1. Three to five samples from each experimental group were analyzed in triplicate. Three to five mice from each experimental group with two lung tissue samples from each mouse were analyzed in triplicate. The data are shown as mean ± standard deviation. * p ≤ 0.05, ** p ≤ 0.01, Mann–Whitney U-test.