Figure 7.

Silencing activity of anti-Timp1 siRNA in vivo. (A) Experimental setup. siTIMP1 or siSCR pre-complexed with cationic liposomes 2X3-DOPE were i.n. administered to mice, then at different time points mice (n = 3) were challenged with LPS and 16 h after induction, lungs were collected for isolation of total RNA. (B) Kinetic of relative Timp1 mRNA level in BALB/c mice receiving siTIMP1_2m or siSCR before LPS challenge. Control—no LPS. Mean values (±SEM) and statistical significance of differences (difference from the siSCR, * p < 0.05), calculated from the results of two independent experiments, are shown, Mann–Whitney U-test.