Table 2.
Primers used for RT-qPCR.
| Gene | Primer/Probe | Sequence 5′–3′ |
|---|---|---|
| Tnf-α | probe | ((5,6)-FAM)-CACAGAAAGCATGATCCGCGACG–BHQ1 |
| forward | CCCTCCAGAAAAGACACCATG | |
| reverse | GCCACAAGCAGGAATGAGAAG | |
| Il6 | probe | ((5,6)-FAM)-TTGTCACCAGCATCAGTCCCAAGAA-BHQ1 |
| forward | AAACCGCTATGAAGTTCCTCTC | |
| reverse | GTGGTATCCTCTGTGAAGTCTC | |
| C3 | probe | ((5,6)-FAM)-ACACCCTGATTGGAGCTAGTGGC-BHQ1 |
| forward | GTTTATTCCTTCATTTCGCCTGG | |
| reverse | GATGGTTATCTCTTGGGTCACC | |
| Serpina3a | probe | ((5,6)-FAM)-ACTGTGGATGGTCTGTGTCAGGC-BHQ1 |
| forward | GGCTTCTATCCTCTGATTGGC | |
| reverse | CCCAGGAATATGTGCTAGTGATG | |
| Dap12 | probe | ((5,6)-FAM)-CCTTCCGCTGTCCCTTGACCTC-BHQ1 |
| forward | GGTGACTTGGTGTTGACTCTG | |
| reverse | GACCCTGAAGCTCCTGATAAG | |
| Adam8 | probe | ((5,6)-FAM)-TCATCTGATACATCTGCCAGCCGC-BHQ1 |
| forward | TATGCAACCACAAGAGGGAG | |
| reverse | ACCAAGACCACAACCACAC | |
| Timp1 | probe | ((5,6)-FAM)-ACTCACTGTTTGTGGACGGATCAGG-BHQ1 |
| forward | CTCAAAGACCTATAGTGCTGGC | |
| reverse | CAAAGTGACGGCTCTGGTAG | |
| Trem2 | probe | ((5,6)-FAM)-ATCTTGCACAAGGTCCCCTCCG-BHQ1 |
| forward | GCTTGGTCATCTCTTTTCTGC | |
| reverse | GTTGAGGGCTTGGGACAG | |
| Mmp9 | probe | ((5,6)-FAM)-TAGCGGTACAAGTATGCCTCTGCC-BHQ1 |
| forward | ACCTGAAAACCTCCAACCTC | |
| reverse | TCGAATGGCCTTTAGTGTCTG | |
| Hprt | probe | ((5,6)-ROX)-CTTGCTGGTGAAAAGGACCTCTCGAA-BHQ2 |
| forward | CCCCAAAATGGTTAAGGTTGC | |
| reverse | AACAAAGTCTGGCCTGTATCC |