Table 6.
Effects of carbohydrase supplementation on intestinal digestive enzymes in hybrid grouper fed different CHO:L ratios diets.
| Groups | CHO:L | Enzyme | Trypsin(U/mg Prot) | Amylase(IU/mg Prot) | Lipase(mU/mg Prot) |
|---|---|---|---|---|---|
| L | 0.91 | 2868.34 ± 117.13 a | 252.22 ± 14.46 a | 1115.49 ± 23.44 b | |
| M | 1.92 | 3997.42 ± 156.84 bc | 553.33 ± 12.79 b | 960.36 ± 26.88 a | |
| H | 3.91 | 3933.64 ± 160.74 bc | 673.75 ± 8.98 c | 920.68 ± 36.56 a | |
| LE | 0.91 | E | 3585.75 ± 166.99 b | 261.73 ± 27.35 a | 1131.23 ± 52.55 b |
| ME | 1.92 | E | 4457.23 ± 155.06 d | 618.85 ± 33.17 c | 1006.00 ± 19.95 a |
| HE | 3.91 | E | 4281.28 ± 178.43 cd | 1000.99 ± 22.07 d | 933.43 ± 27.09 a |
| Means of main effect | |||||
| L | 0.91 | 3227.04 A | 256.98 A | 1123.36 C | |
| M | 1.92 | 4227.32 B | 586.09 B | 983.18 B | |
| H | 3.91 | 4107.46 B | 837.36 C | 927.06 A | |
| No | 3599.80 X | 493.10 X | 998.84 | ||
| Have | 4108.09 Y | 627.19 Y | 1023.55 | ||
| Two-way ANOVA: p-values | |||||
| CHO:L | <0.001 | <0.001 | <0.001 | ||
| Enzyme | <0.001 | <0.001 | 0.137 | ||
| CHO:L*Enzyme | 0.155 | <0.001 | 0.645 | ||
Value is expressed as the means of three replications (n = 3). Averages of different superscripts in the same column (a, b, c, d, etc. Or A, B, C, etc. Or X, Y) are significantly different (p < 0.05). (Dietary CHO:L ratio = A, B, C, etc.; Dietary carbohydrase = X, Y). L, M, H represent the different glycolipid ratios of 0.91, 1.92, 3.91, respectively. L, M, and H represent CHO:L ratios of 0.91, 1.92 and 3.91, respectively. LE, ME, and HE represent CHO:L ratios of 0.91, 1.92, 3.91, respectively, supplemented with the same ratio of carbohydrase; “No” means no enzyme, “Have” and “E” means enzyme.