Table 1.
Oligonucleotides used for this study.
| Genome Target | Nucleotide Sequences | Band Size | Reference |
|---|---|---|---|
| 16S–23S intergenic spacer region (IGS) (Ethiopia soft ticks) |
First round: F: GTATGTTTAGTGAGGGGGGTG R: GGATCATAGCTCAGGTGGTTAG Nested: F: AGGGGGGTGAAGTCGTAACAAG R: GTCTGATAAACCTGAGGTCGGA |
750 bp | [17] |
| Flagellin B (flaB) (Ethiopia soft ticks) |
F: TAATACGTCAGCCATAAATGC R: GCTCTTTGATCAGTTATCATTC |
770 bp | [33] |
| Flagellin B (flaB) (Nigerian livestock) |
First round: F: GATCARGCWCAAYATAACCAWATGCA R: AGATTCAAGTCTGTTTTGGAAAGC Nested: F: GCTGAAGAGCTTGGAATGCAACC R: TGATCAGTTATCATTCTAATAGCA |
350 bp | [34] |
| 16S rRNA (Nigerian livestock) | First round: F: GCGAACGGGTGAGTAACG R: CCTCCCTTACGGGTTAGAA Nested: F: GAGGCGAAGGCGAACTTCTG R: CTAGCGATTCCAACTTCATGAAG |
650 bp | [35] |
| RT-PCR 16S rRNA (All samples) |
F: AGCCTTTAAAGCTTCGCTTGTAG R: GCCTCCCGTAGGAGTCTGG P [FAM] CCGGCCTGAGAGGGTGAACGG |
148 bp | [36] |
| Tick 16S rDNA (All tick samples) |
F: CTGCTCAATGATTTTTTAAATTGC R: CCGGTCTGAACTCAGATCATGTA |
450 bp | [37] |