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. 2023 Jan 11;15(1):209. doi: 10.3390/v15010209

Figure 2.

Figure 2

Cell toxicity assays; MTT assays of cells transfected with empty pcDNA3.1+ vector (mock), pcDNA3.1-NS1-wt, or with pcDNA3.1-NS1-mutants. Relative viability was determined 72 h post-transfection and normalized to pcDNA3.1+ (mock) transfection; (A) MTT assay using NS1-responsive Hep3B cells; relative viabilities of NS1-wt and NS1-T585E expressing cells are highlighted in grey; (B) MTT assay using NS1-unresponsive HepG2 cells (control). Means +/− SEM (n ≥ 5). *: p ≤ 0.05. Grey bars: 100% reference, NS1-wt reference, and most potent mutant, respectively.