Table 1.
Plasmids and Primers used for cloning and mutant design.
| Plasmid | PCR Template | Primers | Expressed Protein |
|---|---|---|---|
| pcDNA3.1+ | original plasmid (Invitrogen) | - | no protein expression |
| pcDNA3.1—NS1 | Witzigmann et al. [3] | - | NS1-wt |
| pcDNA3.1—NS1 (K85Q) | pcDNA3.1—NS1 | p1, p7; p6, p2 | NS1-K85Q |
| pcDNA3.1—NS1 (K257Q) | pcDNA3.1—NS1 | p1, p9; p8, p2 | NS1-K257Q |
| pcDNA3.1—NS1 (S283E) | pcDNA3.1—NS1 | p1, p11; p10, p2 | NS1-S283E |
| pcDNA3.1—NS1 (T435E) | pcDNA3.1—NS1 | p1, p13; p12, p2 | NS1-T435E |
| pcDNA3.1—NS1 (S473E) | pcDNA3.1—NS1 | p1, 15; p14, p2 | NS1-S473E |
| pcDNA3.1—NS1 (T585A) | pcDNA3.1—NS1 | p1, 17; p16, p2 | NS1-T585A |
| pcDNA3.1—NS1 (T585E) | pcDNA3.1—NS1 | p1, p19; p18, p2 | NS1-T585E |
| pcDNA3.1—NS1 (d114) | pcDNA3.1—NS1 | p1, p20; p21, p2 | NS1-d114 |
| pTag-GFP-N | original plasmid (Evrogen) | - | GFP |
| pTag-NS1-GFP | Witzigmann et al. [3] | - | NS1-wt-GFP |
| pTag-NS1(T585E)-GFP | pcDNA3.1—NS1 (T585E) | p1, p3 | NS1-T585E-GFP |
| MCS-BioID2-HA | original plasmid (Addgene) | - | no protein expression |
| pBioID2 | MCS-BioID2-HA | p22, p23 | BioID2 |
| pNS1-BioID2 | pcDNA3.1—NS1 | p4, p5 | NS1-wt-BioID2 |
| pNS1(T585E)-BioID2 | (T585E) | p4, p5 | NS1-T585E-BioID2 |