FIG. 7.
TcsL differential glucosylation of extracts from TcsL- and acid-pulsed TcsL-treated cells. Extracts from CHO cells that had been treated with 1 pmol of TcsL or acid-pulsed TcsL were used in the glucosylation assay to determine if pretreatment under these conditions blocks substrate. Glucosylation assays were carried out with [14C]UDP-glucose and TcsL with the extracts as target substrates. Incorporation of the radiolabeled glucose was determined by autoradiography. (A) Glucosylation of extracts from PBS-treated CHO cells. (B) Glucosylation of extracts from TcsL-treated CHO cells. (C) Glucosylation of extracts from TcsL-treated (acid pulsed; pH 4.0) CHO cells.