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. 2023 Jan 14;24(2):1674. doi: 10.3390/ijms24021674

Figure 1.

Figure 1

NS, NNS, HS, and HNS HBMSC-EV Characterization. Characterization was done with (A) NTA, (B) electron microscopy, and (C) Western blot. CD81, CD9, Alix, and GAPDH were identified in the NS, NNS, and HNS HBMSC-EV types. HSP70 was only identified in HNS HBMSC-EV. Albumin was not identified in any of the HBMSC-EV types, the absence of which is a marker of sample purity. Western blot was used to only qualitatively identify the presence of known HBMSC-EV markers and no statistical analysis or comparisons were performed between HBMSC-EV types. Western blot bands are displayed separately, given the need for wide-ranging exposure times. Given the extremely low protein content of HS HBMSC-EV, electron microscopy and Western blot characterization were not able to detect this HBMSC-EV. Each experiment was performed in triplicates using three independently isolated EV samples (n = 3).