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. 2023 Jan 6;13(2):245. doi: 10.3390/nano13020245

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Fluorescence images of FNP–siRNA complexes in cells at 24 h after incubation (0.05 μM FNP, 100 μM DNA, N/P = 2:1, red: FNP, green: FAM fluorescence). For FNP/FAM-siRNA delivery group: (A) Bright-field channel. (B) Red fluorescence channel showing FNP. (C) Green fluorescence channel showing FAM-siRNA. (D) Fluorescence images of FNP-siRNA complexes ((B,C) merged). For the control group, FAM-siRNA only: (E) Bright-field channel. (F) Green fluorescence channel showing FAM-siRNA.