Figure 6.

HaSCP-2 expression level in the midgut of H. armigera by FNP-RNAi in vivo. Ten 2nd instar larvae were synchronized as a group and fed with FNP-HaSCP-2 siRNA (HaSCP-2 siRNA + FNP group). Midgut tissues from five individual larvae at 3rd, 4th, and 5th instar larvae, respectively, were collected, and the relative HaSCP-2 mRNA expression levels in the larval midgut were determined by qRT-PCR. The 2nd instar larvae were fed with HaSCP-2 siRNA and DEPC-treated ddH₂O (Wild Type group), respectively, were used as the control. Data are mean ± SD. Asterisk “**” indicates significance at p < 0.01 level.