Table 1.

Primers for quantitative real-time PCR and siRNA.

Primer Name	Sequence (5′→3′)	Length (Base)	Tm	Efficiency
Q HaSCP-2F	GAGAAAGTACGAGGCATCTATGG	23	58	99%
Q HaSCP-2R	ATCGCTGATGGTGAAGGTG	19	55
Q rpS3A-F	CCTCGTGAGGCCAACAT	17	54	98%
Q rpS3A-R	TGATGGCACGGACCTGAGT	19	57
HaSCP-2 siRNA	Sense:5′-GCAAAUGAGCUGAUCACAUTT-3′ Antisense: 5′-AUGUGAUCAGCUCAUUUGCTT-3′	21
FAM-siRNA (Negative control)	Sense:5′-UUCUCCGAACGUGUCACGUTT-3′ Antisense: 5′-ACGUGACACGUUCGGAGAATT-3′	21