Table 1.
Calculated with OriginPro 2015 program from radioligand assay (see the respective inhibition curves in Figure 4) the binding parameters—IC50 and Hill slope values—for recombinant sea anemone peptides on muscle-type T. californica and neuronal human α7 nAChRs. Data are shown as mean (95% confidence interval).
| Peptide | [125I]α-Bgt-Competitive Binding Assay | |||
|---|---|---|---|---|
| T. californica α1β1γδ nAChR | Human α7 nAChR | |||
| IC50 (μM) | Hill Slope | IC50 (μM) | Hill Slope | |
| Ms11a-1 | 0.408 (0.395–0.421) | 0.96 (0.94–0.99) | 14.16 (13.48–14.87) | 1.18 (1.11–1.25) |
| Ms11a-2 | 1.080 (0.947–12.28) | 0.82 (0.75–0.89) | 14.13 (12.43–16.07) | 0.80 (0.69–0.91) |
| Ms11a-3 | 0.256 (0.236–0.277) | 0.98 (0.91–1.05) | 19.81 (18.44–21.28) | 2.37 (2.00–2.75) |
| Ms11a-4 | 14.95 (13.46–16.61) | 1.14 (1.02–1.26) | >45 | - |