Figure 3.

(A) Nominal recovery (ng/mL) from exposure of micro-O-Rings loaded with 400 ng/mL each PAH in mixture ([10 µg/mL] total) to media without cells measured at 1, 8, and 24 h (red dashed lines). Stability in recovery from micro-O-rings was observed for most compounds after 8 h. (B) Nominal recovery (ng/mL) from exposure of MAF prepared with 400 ng/mL each PAH, [10 µg/mL] total, to media without cells measured at 1, 4, 8, and 24 h. Longer exposure times yielded less recovery, stabilizing by 8 h for most compounds. (C) Nominal recovery (ng/mL) from exposure to DMSO extract prepared with 15 ng/mL each PAH, [0.375 µg/mL] total, to media without cells measured at 1, 4, 8, and 24 h. Recovery stabilized by 8 or 24 h for most compounds. (D) Strong positive linear correlation observed between fractional recovery from DMSO extract (x-axis) and MAF (y-axis) exposures to media over 1 (R² = 0.81), 4 (R² = 0.91), 8 (R² = 0.93), and 24 h (R² = 0.89) timepoints (varied by size and shade of gray).