Figure 1.
Fe2+ binding to FXN. (A) overlap of three different 15N,13C-CON spectra (as shown in the legend), showing the peak assignments for the backbone amide groups. (B) Intensity ratio normalized to the urea signal as a function of the amino acid for two different experimental conditions, as indicated in the legend. (C) Chemical shift perturbation (CSP) as a function of the amino acid for the same dataset. (D) Structural representation of the intensity variations at 1 equivalent (left) and 3 equivalents (right) of FeCl2. The color code reflects the intensity variations and it is proportional to the standard deviation scale from the average value, as indicated in the bar legend (x meaning average value). The gray lines correspond to the average values for all the residues.