FIG. 6.

DNA-mediated activation of the MAP kinases ERK1 and -2 and their inhibition by geldanamycin in murine macrophages. J774 cells were pretreated with or without 1 μg of geldanamycin per ml for 1 h and then stimulated with 10 μg of CpG ODN (CpG) or E. coli DNA (EC) per ml or 1 μg of LPS per ml in the presence or absence of geldanamycin for 30 min. Cell lysates were analyzed for the presence of phosphorylated ERK1 and -2 by immunoblotting. Membranes were exposed using ECL, the images were scanned, and optical densities were determined. Values are presented as the fold increases in optical density against that of a medium control without geldanamycin treatment (Med). The data are representative of four separate experiments.