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. 2022 Dec 22;16(1):14. doi: 10.3390/ph16010014

Figure 5.

Figure 5

Compound DEA (1) induces antioxidant factor expression in the ALD cell model. (A) Effects of 10 µM, 30 µM DEA (1), and 30 µM ML334 on Nrf1, NQO1, HO–1, Keap1, and Nrf2 protein levels in LO2 cells after 8 h treatment. (B–D) Quantification analysis of Nrf1 (B), NQO1 (C), and HO–1 (D) in WB. (E–I) Effects of DEA (1) and ML334 on (E) SOD2, (F) NQO1, (G) HO–1, (H) Nrf1, (I) PGC–1α mRNA levels in 0.3% EtOH–induced LO2 cells. Data are represented as mean ± SD. * p < 0.05, *** p < 0.001 vs. control group. The data represent the standard deviations of the results from three independent experiments. EtOH: Ethyl alcohol. Nrf1: Nuclear respiratory factor–1; NQO1: NAD(P)H dehydrogenase [quinone] 1; PGC–1α: Peroxisome proliferator–activated receptor γ coactivator 1α; SOD 2: Superoxide dismutase 2.