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. 2023 Jan 6;24(2):1118. doi: 10.3390/ijms24021118

Figure 2.

Figure 2

NETs accelerate the migration of CRC cells through the release of NE in vitro. (A) Cell proliferation assay using HCT116 and HT29 cells. Cells were incubated with NET-conditioned medium (NET-CM) or neutrophil control medium and were analyzed at 0, 24, 48 and 72 hours. The absorbance at time 0 was normalized to 1. Mean: bars ± standard error of the mean (SEM). n = 3. ns: not significant with Student’s t test. (B) Cell migration assay using scratch wound healing. HCT116 and HT29 cells were incubated with NET-CM or neutrophil control medium and were treated with 100 µM of sivelestat. Representative images are shown. Scale bars, 500 µm. (C) Wound width was measured at 0, 24 and 48 hours. Normalized gap width at hour 0 was 1. Mean: bars ± standard deviation (SD). n = 6. * p < 0.05 with Student’s t test.