Figure 3.

The GBM (red, λ_ex = 555 nm) is characterized by reducing intranasal delivery of liposomes to the brain and their removal to the dcLNs: (a)–(d) representative confocal images of liposomes (green) spreading in dorsal part of the brain and their accumulation in the dcLN in the GBM group (a,c) and in the HP (b,d), the blood vessels were filled with Evans Blue (red, λ_ex = 640 nm); the quantitative analysis of fluorescent signal from liposomes (a.u.) in the brain (e) and in the dcLNs (f) in the GBM group and in the HG groups, n = 5 in each group, * p < 0.05, and ** p < 0.01 the Mann–Whitney–Wilcoxon test, the outliers of experimental data are plotted using the + symbol.