Table 4.

Comparison of the advantages and disadvantages of commonly used EV isolation techniques [148,149].

	Ultracentrifugation	Polymer Based Precipitation	Size Exclusion Chromatography	Ultrafiltration	Immunoaffinity
Advantages	High purity Can collect different size EVs	No specialised equipment Quick High yield High throughput	High purity Low cost Quick	Low cost Quick	Can be used to separate EVs of different origins High purity
Disadvantages	Low yield Specialised equipment Requires large sample Low throughput	Low purity	Low yield	EV clogging and trapping Low yield Low purity	Costs EV markers require optimisation Elution steps may damage EV structure