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. 2023 Jan 14;2023:9966355. doi: 10.1155/2023/9966355

Figure 4.

Figure 4

TBM protected H9c2 cells from DOX-induced apoptosis. (a, b) TUNEL staining labeled DNA breaks in H9c2 cells and its statistical analysis (n = 3). (c, d) Flow cytometry was also used to evaluate the apoptosis of H9c2 cells (n = 6). (e, f) Mitochondrial membrane potential (MMP) was determined by JC-1staining (n = 3). (g, h) Flow cytometry was used to estimate MMP in each group (n = 3). (i, j) Bax and Bcl-2 mRNA levels tested by qPCR (n = 5). (k–o) The expression of BAX, BCL-2, and C-caspase3 at protein level determined by western blotting in vitro (n = 3). Values indicate the mean ± SD. ∗P < 0.05 versus the CON group and the TBM group; #P < 0.05 versus the DOX group; ns (not significant) versus the CON group.