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. 2023 Jan 14;2023:9966355. doi: 10.1155/2023/9966355

Figure 9.

Figure 9

TBM prevented DOX-evoked cellular damage by upregulating SIRT3 in H9c2 cells. (a, b) si-SIRT3 inhibited the expression of SIRT3 as determined by western blotting (n = 4). (c–h) The expression of SIRT3, P-P65, T-P65, IL-6, IL-1β, and TNF-α was estimated by western blotting in H9c2 cells (n = 3). (i–m) Western blotting was used to detect the expression of Nrf2, HO-1, and NQO1 in vitro (n = 3). (n, p) si-Sirt3 partially reversed the inhibitory roles of TBM in DOX-induced ROS production (n = 4). (o, q) Flow cytometry analysis demonstrated that SIRT3 inhibition blunted the beneficial effects of TBM on DOX-evoked apoptosis (n = 6). (r–v) Western blotting was used to test the expression of apoptosis related proteins (BAX, BCL-2, and C-Caspase3) in H9c2 cells after si-Sirt3 treatment (n = 3). Values indicate the mean ± SD. ∗P < 0.05 versus the CON group and the TBM group; #P < 0.05 versus the DOX group; ns (not significant) versus the CON group; &P < 0.05 versus the DOX + TBM + siSIRT3 group.