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Ki-67 staining of encapsulated MDA-MB-231 cells within 3D bioprinted constructs. Cells were stained using anti-Ki-67 antibodies visualized with Alexa Fluor 546, and Hoechst 33,342 (red color represents cells positive to ki-67; blue color represents all cells). Cells were imaged using the laser scanning confocal microscope. Scale bar, 50 μm (enlarged image, scale bar, 30 μm).
