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. 2023 Jan 21;13:1211. doi: 10.1038/s41598-023-28286-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Middle panels visualize the MDA-MB-321 growth within the 3D hydrogel construct in silico; yellow represents proliferating cells; red represents non-proliferating cells; black represents dead cells. The right and left panels represent MDA-MB-231 cells encapsulated in 3D hydrogel constructs observed by a phase-contrast microscope on day 0, day 4, day 7, and day 11: scale bar, 50 μm.