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. 2023 Jan 21;13:1215. doi: 10.1038/s41598-023-27782-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression levels of mouse α-tubulin genes in the developing brain are perturbed in the R402H Tuba1a (modified) line. (a–d) mRNA expression levels of Tuba1a (a), Tuba1b (b), Tuba1c (c) and Tuba4a (d) were assessed by qPCR in E16.5 wild-type (grey), heterozygous (light green), and homozygous R402H^wt/R402H^wt (dark green) embryos. We found a slight reduction in the expression levels of Tuba1a levels between wild-type and heterozygous animals (n = 5, +/+ vs. R402H^wt/+ P < 0.05). (e–h) mRNA expression levels of Tuba1a (e), Tuba1b (f), Tuba1c (g) and Tuba4a (h) were assessed by qPCR in E16.5 wild-type (grey), heterozygous (light blue), and homozygous R402H^mod/R402H^mod (dark blue) embryos. There was a significant reduction in Tuba1a levels in both heterozygous and homozygous animals (n = 5, +/+ vs. R402H^mod/+ P < 0.0001; +/+ vs. R402H^mod/R402H^mod P < 0.0001 and R402H^mod/+ vs. R402H^mod/R402H^mod P < 0.0001). Error bars show mean ± s.e.m.. Two-way repeated measures ANOVA with Tukey’s test for multiple comparisons; n.s.—not significant; *P < 0.05; ****P < 0.0001.