Fig. 3. Effect of corticosterone administration at the non-physiological timing.

a Diurnal change in serum corticosterone levels in normal mice at our facility. Each data series represents the mean ± SEM and 4 mice per time point. b A schematic diagram of the experiment that mice were orally administered corticosterone (CORT) for 7 consecutive days at ZT 1 and sacrificed every 4 h on day 8 (ZT 4,8,12,16,20,24). c Relative mRNA accumulation of Bmal1, Per2, and Rev-erbα in the liver and bladder from mice vehicle (solid line) and CORT (dash line) at ZT 1. Each data series represents the mean ± SEM and 5 mice per time point. Differences were determined by two-way ANOVA and Sidak’s multiple comparisons test. *p < 0.01 v.s. vehicle (ZT: Zeitgeber time).