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. 2023 Jan 21;6:81. doi: 10.1038/s42003-023-04464-3

Fig. 5. Effect of bilateral adrenalectomy and corticosterone administration at the non-physiological timing.

Fig. 5

a A schematic diagram of the bilateral adrenalectomy (ADx) performed on Day 1 and corticosterone administered orally for 7 days at ZT 1 from Day 8 after ADx and sacrifice on Day 15 (ADx + CORT). b Relative mRNA accumulation of Bmal1, Per2, and Rev-erbα in the liver and bladder from ADx + vehicle (solid line) and ADx + CORT (dash line). Each data series represents the mean ± SEM and 5 mice per time point. Differences were determined by two-way ANOVA and Sidak’s multiple comparisons test. *p < 0.01 v.s. ADx + vehicle. c Temporal mRNA accumulation of clock genes in the bladder from ADx + vehicle (solid line) and ADx + CORT (dash line) by analyzing RNA-seq. Each data series represents the 3 mice per time point.