Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 21;14:349. doi: 10.1038/s41467-023-35965-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a RT-qPCR analysis of transcript levels of E-CAD, N-CAD, SNA1, SNAI2, TWIST1 and VIMENTIN in CTRLsh and GPC4sh5-c10 029 hiPSCs differentiated for 5 days into DE or ME. Top panel: DE differentiation and bottom panel: ME differentiation. Data were normalized to the mean of CTRLsh ΔCt at d0 and represented as mean ± SEM, n = 3. b, c Immunofluorescence analysis of N-CAD (magenta), E-CAD (green), SOX17 (cyan, DE) or PDGFRa (cyan ME) in WT, CTRLsh, GPC4sh5-c10 and GPC4sh2-c3 029 hiPSCs differentiated for 3 days into DE (b) or ME (c), n = 2. Outlined areas highlight the DE and ME differentiated cells, scale bar: 100 μm. Statistical analysis for the overall figure: (a) two-way ANOVA followed by Sidak’s multiple comparison test. P-values: (***) <0.001, (**) <0.01, (*) <0.05. For all panels “n” corresponds to the number of biological replicates unless stated otherwise. Source data are provided as a Source Data file.