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. 2023 Jan 21;14:349. doi: 10.1038/s41467-023-35965-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative 2D gastruloids immunostained for DAPI positive nuclei (blue), SOX2 (green) and BRACH (magenta) proteins 48h after the start of BMP4 stimulation (colony diameter 700 μm; scale bar 100 μm). b Quantification of SOX2 and BRACH radial profiles of expression in human 2D gastruloids of panel a. For each stain, radial profiles are normalized by the maximum of the control profile. Shaded areas represent the 1st and 3rd quartiles of the individual colony profiles with a line at the mean. CTRLsh: n = 64, GPC4sh n = 67 colonies from three biological replicates. c Representative 2D gastruloids immunostained for DAPI positive nuclei (blue), SOX2 (green) and SOX17 (magenta) 48h after the start of BMP4 stimulation (colony diameter 700 µm, scale bar 100 μm). d Quantification of SOX2 and SOX17 radial profiles of expression in human 2D gastruloids of panel c. For each stain, radial profiles are normalized by the maximum of the control profile. Shaded areas represent the 1st and 3rd quartiles of the individual colony profiles with a line at the mean. CTRLsh: n = 60, GPC4sh n = 62 colonies from 3 biological replicates. Source data are provided as a Source Data file.