Fig. 2. Determination of differential gene expression in context with T cell activation and numerical comparison of deregulated genes.

A Following T cell activation a total of 30,158 genes was analyzed for time-course RNA expression changes. There were 535 genes with a median log₂ fold change (FC) ≥0.5 or ≤−0.5 during the time-course. The comparison between the PD and HC groups showed 454 genes with a median log₂ FC ≥0.5 or ≤−0.5 and 175 genes with a significantly altered expression (p-values ≤ 0.05 adjusted by Benjamini & Hochberg). The 172 genes that showed both a significantly altered expression and a median log₂ FC ≥0.5 or ≤−0.5 are referred to as core deregulated genes. B, C The Venn diagrams provide a numerical summary of genes that were deregulated at different time windows upon T cell activation. The diagram (B) includes the genes with a median log₂ FC ≥0.5 or ≤−0.5 and the diagram (C) the genes with an adjusted p-values ≤ 0.05.