TABLE 2.
Lesion formation by wild-type and mutant H. ducreyi strains in the temperature-dependent rabbit modela
| Strain | Inoculum size (CFU) | Mean (±SD) lesion scoreb
|
P valuec | ||
|---|---|---|---|---|---|
| Day 2 | Day 4 | Day 7 | |||
| 35000 (wild type) | 105 | 4.00 (0) | 4.00 (0) | 4.00 (0) | |
| 35000.301 (cdtA mutant) | 105 | 4.00 (0) | 4.00 (0) | 4.00 (0) | |
| 35000.302 (cdtB mutant) | 105 | 3.88 (0.35) | 4.00 (0) | 4.00 (0) | |
| 35000.303 (cdtC mutant) | 105 | 4.00 (0) | 4.00 (0) | 4.00 (0) | |
| 35000 (wild type) | 104 | 3.50 (0.53) | 4.00 (0) | 4.00 (0) | |
| 35000.301 (cdtA mutant) | 104 | 3.38 (0.52) | 3.75 (0.46) | 4.00 (0) | 0.0796 |
| 35000.302 (cdtB mutant) | 104 | 3.25 (0.46) | 3.38 (0.52) | 3.75 (0.46) | 0.0256 |
| 35000.303 (cdtC mutant) | 104 | 3.63 (0.52) | 3.75 (0.46) | 4.00 (0) | 0.6845 |
Eight rabbits were used in this experiment.
Lesion characteristics were scored as follows: 0, no change; 1, erythema; 2, induration; 3, nodule; 4, pustule or necrosis.
P values were calculated for the difference between wild-type and mutant strain lesion scores. P values were calculated using the lesion scores from the 104-CFU inoculum size on 3 days. Using the Bonferroni correction necessitated by the number of comparisons between strains in this analysis, the P value needed for significance is 0.0083. Therefore, there was no significant difference in lesion scores produced by the three isogenic mutants when they were compared in a pairwise manner.