Table 2.
Cysteine residues in Stat3 protein that were modified by azetidine compounds and detected by MS.
Compound name | Cys residue | m/z | Mr(expt) | Mr(calc) | ppm | Score | Expect | Peptide | Experimental reproducibility |
---|---|---|---|---|---|---|---|---|---|
H098 | C328 | 920.3475 | 1838.6804 | 1838.6800 | 0.23 | 39 | 0.0002 | F.VVERQPCMPM.H + 2 Oxidation (M); STAT3-H098 (C) | 3 out of 3 |
C426 | 791.2959 | 1580.5772 | 1580.5687 | 5.38 | 22 | 0.0093 | N.GGRANCDASL.I + STAT3-H098 (C) | 3 out of 3 | |
H182 | C468 | 1121.0018 | 2239.9891 | 2239.9880 | 0.51 | 37 | 0.00036 | H.SLPVVVISNICQMPN.A + STAT3- H182 (C) | 1 out of 2 |
C542 | 711.2601 | 1420.5057 | 1420.4992 | 4.60 | 18 | 0.046 | Y.SGCQITW.A + STAT3-H182 (C) | 2 out of 2 |