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. Author manuscript; available in PMC: 2023 Jan 22.
Published in final edited form as: J Am Soc Mass Spectrom. 2021 Oct 26;32(12):2728–2737. doi: 10.1021/jasms.1c00081

Table 1.

Spraying Parameters for All Eight Spraying Conditions Evaluated

kidney
section		 matrix solvent matrix
concentration
(mg/mL)		 solvent flow rate
(mL/min)		 number of
passes		 nozzle speed
(mm/min)		 nozzle
temperature
(°C)		 spraying
pattern		 nitrogen
pressure (PSI)		 tray
temperature
(°C)		 density of matrix
deposited (mg/mm2)		 linear flow rate
(mL/mm)
1 DHB 70% MeOH/30% H2O 40 0.100 13 1920 75 CC 10 48 0.0135 5.21 × 10−5
2 DHB 70% MeOH/30% H2O 40 0.100 13 1920 75 CC 10 ambient 0.0135 5.21 × 10−5
3 DHB 70% MeOH/30% H2O 40 0.100 8 1200 75 CC 10 ambient 0.0133 8.33 × 10−5
4 DHB 70% MeOH/30% H2O 40 0.160 8 1920 75 CC 10 48 0.0133 8.33 × 10−5
5 DHB 70% MeOH/30% H2O 40 0.160 5 1200 75 CC 10 ambient 0.0133 1.33 × 10−5
6 DHB 70% MeOH/30% H2O 40 0.160 5 1200 75 CC 10 48 0.0133 1.33 × 10−5
7 DHB 70% MeOH/30% H2O 40 0.160 8 1920 75 CC 10 ambient 0.0133 8.33 × 10−5
8 DHB 70% MeOH/30% H2O 40 0.100 8 1200 75 CC 10 48 0.0133 8.33 × 10−5