Skip to main content
. Author manuscript; available in PMC: 2023 Sep 20.
Published in final edited form as: Anal Chem. 2022 Sep 9;94(37):12559–12564. doi: 10.1021/acs.analchem.2c03549

Figure 1.

Figure 1.

LC-PRM method for uncovering alterations in expression of epitranscriptomic RWE proteins elicited by genetic ablations of m6A writer and eraser proteins. (a) Workflow of LC-PRM analysis coupled with the use of SIL peptides as internal or surrogate standards for profiling epitranscriptomic RWE proteins in HEK293T and the isogenic ALKBH5−/−, FTO−/−, and METTL3−/− cells. (b) A Venn diagram depicting the numbers of quantified RWE proteins in HEK293T, ALKBH5−/−, FTO−/−, and METTL3−/− cells, compared with those deposited in the PRM library. The Venn diagram was designed using the webtool at https://bioinformatics.psb.ugent.be/webtools/Venn/.