Figure 1.

Human NAA30 rescues Arl3 localization and Nfs^–phenotypes of naa30Δ yeast.A, live imaged Arl3-GFP yeast of the indicated genotypes. Shown are representative zoomed fields (scale bar represents 2.5 μm), based on images from > 100 yeast cells from approximately 20 random fields of view per genotype. B and C, cells were cultured in medium with either glucose (B) or glycerol (C) as the sole carbon source and the cell density was measured by optical density (A₆₀₀). The bar charts show the increased cell density, A_600, from initial to late exponential growth phase. All data shown represent the mean of three technical replicates from two biological replicates (sister clones) per genotype (n = 6 in total per genotype), and all error bars show SD. Data were analyzed by a two-tailed t test with unequal variance. ∗∗∗ indicate p-value <0.0005, ∗∗∗∗ indicate p-value <0.00005 and other comparisons to WT (+hNAA50 and +hNAA60) were non-significant.