FIG. 2.

A/J mice produce antibodies specific for cardiac proteins after infection with T. cruzi or immunization with myosin. Serum samples from four groups of A/J mice (five mice per group) obtained 21 d.p.i. with PBS (uninfected group), with PBS-CFA (unimmunized group), with 10⁴ T. cruzi trypomastigotes (infected group), or with myosin-CFA (immunized) were pooled for use in Western blot and ELISA analyses. (A) A/J mouse heart homogenate (top) or purified cardiac myosin (bottom) was resolved by SDS-PAGE, transferred to nitrocellulose, and blotted with 1:100 dilutions of sera from groups 1 to 4. The arrowhead indicates the position of cardiac myosin heavy chain. (B) Purified regulatory myosin light-chain kinase (MLCK) and myelin basic protein (MBP) were resolved by SDS-PAGE, transferred to nitrocellulose, and blotted with 1:100 dilutions of sera from the groups. A Coomassie blue-stained gel is also shown. (C) Sera from the four groups were tested for myosin-specific IgG by ELISA. Each datum point represents the average for three wells (standard error of the mean, <0.09).