FIG. 3.

T. cruz-infected mice and myosin-immunized mice display myosin-specific cellular autoimmunity. Myosin-specific DTH was determined by a 24-h ear swelling assay. Error bars indicate standard errors of the means. (A) Time-course of myosin-specific DTH in T. cruzi-infected mice. Myosin-specific DTH was measured in eight mice infected with 10⁴ T. cruzi trypomastigotes (infected) and in five mice injected with PBS (uninfected) at each d.p.i. For comparison, at 21 d.p.i., myosin-specific DTH was measured in eight myosin-immunized (immunized) mice and five PBS-CFA-immunized (unimmunized) control mice. T. cruzi-infected mice developed significant myosin-specific DTH as early as 7 d.p.i. (B) T. cruzi-specific DTH was also measured in eight infected mice and five uninfected mice at 21 d.p.i. ∗, P of <0.001; †, P = 0.002 compared to the respective controls.